Production of Diphenols by Polyphenol Oxidase from Mushroom and Mung Bean (Vigna mungo).
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چکیده
منابع مشابه
Purification and Characterization of the Main Isozyme of Polyphenol Oxidase in Mung Bean (Vigna mungo) Seedlings.
Pe]ypheno] oxidase (monophenol dLhydrexyphenylalanine' oxygen ox]doTeductase/ EC 1 14.1S t; PPO) hus been found in most higher plunts. although its ro]e has not yet becn clearly cstab]]shed. When the extracts frem var]ous organs of' mung bean seedlings were unalyzecl by :soelectric f'ocustng, several bancls of PPO ttet]vity were dctectcd. Among these isozyrnes, the one with pl S,4 showed the hi...
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Two forms of Fd-NADP+ oxidoreductase (FNR) isoproteins have been purified and characterized from the first foliage leaves of 5d-old mung bean (Vigna radiata). They could be separated by either Mono Q HR 515 or ferredoxin (Fd)-Sepharose 48 affinity columns. Based on immunoblot analysis and N-terminal amino acid sequences, one form resembles the FNR purified from photosynthetic tissues of higher ...
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Polyphenoloxidase was purified from chloroplasts of broad bean leaves (Vicia faba L.) to apparent homogeneity. The enzyme was composed of two proteins with an apparent mass of 65 and 68 kilodaltons after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isolated enzyme contained covalently attached carbohydrates and bound concanavalin A, Phaseolus vulgaris erythroagglutinin, and Ri...
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The subtilisin inhibitor (MBSI-A) from the mung bean (Vigna radiata (L.) Wilczek) seed has been purified to homogeneity. MBSI-A consists of a single polypeptide chain of 119 residues, with a high content of glutamic acid/glutamine, aspartic acid/asparagine, valine, threonine, and proline (19, 12, 10, 9, and 8 residue percent, respectively). MBSI-A is a potent inhibitor of subtilisin Carlsberg, ...
متن کاملPurification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified...
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ژورنال
عنوان ژورنال: NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI
سال: 1996
ISSN: 1341-027X,1881-6681
DOI: 10.3136/nskkk.43.875